Features

Cellometer Spectrum

Image Cytometry System for 20 µl Cell-Based Assays

Why Cellometer Spectrum?

20 µ Cell-Based Assays
Unique Algorithms for Advanced Cell Analysis
User-changeable Fluorescent Modules

Introduction to the Cellometer Spectrum

Whether you are looking for an affordable benchtop cytometer, or to improve your current Flow cytometry analysis, the Spectrum Image cytometry system will exceed your expectations not only in performance, data analysis and affordability, but also in its ease of use and application versatility.

All-in-One System

Basic cell counting, primary cell viability, and cell-based assays.

Dual-Fluorescence for Accurate Primary Cell Viability

No interference from red blood cells. Analyze bone marrow, peripheral blood, and cord blood without lysing.

Unique Algorithms for Advanced Cell Analysis

Determine concentration and viability of hepatocytes, adipocytes, and other sophisticated cell types.

Fast Results

Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

Simple 20 µl Cell-Based Assays

GFP | Apoptosis | Cell Cycle | Cell Mediated Cytotoxicity | Cell Counting & Viability

Accurate Cell Counting
Versatile Analysis and Applications
Pre-qualified Reagents
Pre-defined Instrument Settings
Assay-Specific data templated
Export to FCS Express* (Flow like data analysis)

Cell Types for Many Research Areas

Cellometers are optimized for primary cell analysis

PBMCs
Stem Cells
Adipocytes
Neural Cells
Hepatocytes
Dendritic Cells

Epithelial Cells
Keratinocytes
Lymphocytes
Splenocytes
Monocytes

Clinical Immunology: PBMCs
Diabetes / Obesity: Adipocytes
Immunotherapy: Leukocytes
Microbiology: Yeast (Spectrum 10x)
Oncology: Cell Lines
Regenerative Medicine: Stem Cells
Toxicology: Hepatocytes
Transplantation: Nucleated Cells
Vaccine Development: Splenocytes

Accurate Cell Counting

Bright Field

Stained AO+ Cells

Counted AO+ Cells

Automatic identification of Acridine Orange (AO) stained cells (shown above)
Image and count up to 1.51x10⁷ cells/mL
Image acquisition and analysis: 30 seconds/sample for a 3-channel assay
Versatile platform - use for routine cell counting and complex cell-based assays

Export to FCS Express* for Flow-Like Data

Counted PI+ Cells

Contour Population Plot

Export image data into flow cytometry software: FCS express
Simple work flow: No fluid- stream, No PMT voltages, No forward/side scatter
Easily perform data analysis using pre-designed templates
Quickly plot cell population data as a: histogram, scatter plot, dot plot or contour plot

* FCS Express Flow Cytometry software is a product of De Novo Software and is included with the Cellometer Spectrum

Cell Population	% of Gated Cells	Concentration (10^6 cells/mL)
Total	100	9.2
Sub G1	4.7	0.1
G0/G1	58.0	5.8
S	13.4	1.3
G2/M	23.9	1.9

User-Changeable Fluorescence Optics Modules*

Cellometer assays use specific optic modules for maximum performance and discrimination between fluorescence channels. Standard modules are listed in the table below. Custom fluorescence optics modules are also available.

	Fluorophores	Nucleic Acid Stains
VB-450-302 Ex: 375 nm Em: 450 nm	AlexaFluor® 350 CFP	DAPI Hoechst 33342 Hoechst 33258
VB-535-402 Ex: 475 nm Em: 535 nm	GFP Calcein FITC AlexaFluor® 488	AO (acridine orange) SYTO®9, SYTO®13 SYTOX®Green SYTO®BC
VB-595-502 Ex: 525 nm Em: 595 nm	AlexaFluor® 546 AlexaFluor® 555, Cy3® PE (R-phycoerythrin) Rhodamine B	PI (propidium iodide) EB (ethidium bromide) SYTOX® Orange
VB-660-502 Ex: 540 nm Em: 660 nm	RFP 7-AAD Nile Red	PI (propidium iodide) EB (ethidium bromide)
VB-695-602 Ex: 630 nm Em: 695 nm	AlexaFluor® 647, Cy5® APC (allophycocyanin)	SYTOX® Red

*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Nexcelom technical support regarding compatibility of other reagents.
Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.