Cellometer and Celigo Image Cytometers View all Products →

Nexcelom’s image cytometers give researchers increased control of their samples and the data generated from their cell-based assays. The Cellometer Vision CBA system requires 20 µL of sample in suspension and is a simple, quick solution for assays such as cell cycle, apoptosis, GFP transfection and surface marker analysis. The Celigo offers a plate-based high-throughput solution for adherent and suspension cells, eliminating the need to trypsinize cells and is ideal for a broad range of applications including 3D multicellular tumor spheroids, proliferation, cell line development, colony counting, wound healing, and more.

Cellometer Vision CBA

Advanced imaging with 2 interchangeable fluorescent optics modules for simple, single sample 20µl cell-based assays with flow-like data output.

Celigo Image Cytometer

A micro-well, plate-based multi-channel bright field and fluorescent imaging cytometer for 2D and 3D culture using both adherent and suspension cells.

Cellometer and Celigo Image Cytometers at a Glance

Cell / Sample Type
	Vision CBA	Vision CBA (10x)	Celigo BF	Celigo 4 Channel	Celigo 5 Channel
Cell Line	X		X	X	X
Cultured Primary Cells	X		X	X	X
Algae		X
Platelets		X
Low Concentration Cell Lines	X		X	X	X
Yeast (Clean Sample)		X
Yeast (Messy Sample)		X
Primary cells (Messy Sample*)	X			X	X
PBMCs, Splenocytes, Stem Cells	X			X	X
Hepatocytes	X			X	X
Adipocytes***	X		X	X	X
Cell-Based Assay **	X	X	X	X	X
Apoptosis (Annexin V-FITC/PI)	X	X		X	X
Apoptosis (Caspase Activity)	X	X		X	X
Autophagy (CytoID-green)	X	X
Cell Proliferation (CFSE)	X	X		X	X
Cell Cycle (PI)	X	X		X	X
GFP Transfection	X	X		X	X
RFP Transfection	X	X		X	X
Mitochondrial Potential (JC-1)	X	X		X	X
Multi-drug Resistance (ABC Transporter)	X	X		X	X
Surface Marker Analysis	X	X		X	X
Vitality (Calcein-AM/PI)	X	X		X	X
Image Cytometry**	X	X		X	X
Imaging Capabilities
Fluorescence Intensity Measurements	X	X		X	X
Detection of Weak Fluorescence	X	X		X	X
Configuration
Objective Magnification	5X	10X
Bright field	X	X	X	X	X
Contains Single Fluorescence Optics Module
Contains Dual Fluorescence Optics Module	X	X
3 Fluorescent Channels				X
4 Fluorescent Channels					X
Desktop Work Station Included			X	X	X
Optional Satellite Work Station Available			X	X	X
User-changeable optics module(s)	X	X
Software Features
Software for analysis of clumpy and irregular-shaped cells	X	X	X	X	X
Mean cell diameter and cell size distribution	X	X	X	X	X
Sample adjustment calculator to determine sample volume for desired cell number / concentration	X	X
Cell type wizard for creating new cell type parameters	X	X
IQ / OQ Option	X	X
GLP/GMP Audit Control Option	X	X
Specialized algorithms for analysis of adipocytes***	X
Specialized algorithms for analysis of hepatocytes	X
FCS Express Software for flow-like data reporting for cell-based assays	X	X		X	X
FCS Express Software for Image Cytometry	X	X		X	X
Performance Criteria
Analysis time	5-15 sec.	5-15 sec.
Cell size range	5-300µm	0.5-15µm
Sample volume	20 µl	20 µl

*A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.
** FCS Express license must be purchased in order to perform Cell Based Assay or Image Cytometry analysis
*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter